Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1215-1219.doi: 10.3969/j.issn.1673-8225.2012.07.018

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Effect of exercise on number and activity of circulating endothelial progenitor cells in healthy subjects

Yang Zhen1, Lai Guang-hua1, Xia Wen-hao1, Luo Chu-fan2, Cheng Fei1, Liao Yan-hong1, Wang Jie-mei1, Chen Long1, Liao Xin-xue1, Jin Ya-fei1, Tao Jun1   

  1. 1Department of Hypertension & Vascular Disease, 2Department of Cardiac Intervention, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-08-06 Revised:2011-11-09 Online:2012-02-12 Published:2012-02-12
  • Contact: Tao Jun, Doctor, Professor, Doctoral supervisor, Department of Hypertension & Vascular Disease, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China taojungz@yahoo.com
  • About author:Yang Zhen☆, Doctor, Attending physician, Department of Hypertension & Vascular Disease, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China yangzhen107107@yahoo.com.cn
  • Supported by:

    National Natural Science Foundation of China, No. u0732002*, 30800215*, 30770895*; Doctor Starting Project of Natural Science Foundation of Guangdong Province, No. 8451008901000793*; Science and Technology Project of Guangzhou, No. 2007Z3-E0241*

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Abstract:

BACKGROUND: Whether exercise can influence the number and activity of endothelial progenitor cells (EPCs) is still unclear.
OBJECTIVE: To observe the effect of exercise on number and activity of circulating EPCs in healthy subjects.
METHODS: Twelve male healthy volunteers underwent acute dynamic treadmill exercise with (9.3±2.1) minutes. Flow cytometry analysis was used to evaluate the number of CD34 and KDR double-positive labeled circulating EPCs before and after exercise, and acetylated-low-density lipoprotein (LDL) and lectin fluorescent staining method were used to evaluate the number of cultured EPCs. The adhesion, migration and proliferation of EPCs were detected. In addition, the nitric oxide (NO), vascular endothelial growth factor (VEGF) and granulocyte-macrophage colony stimulating factor (GM-CSF) levels in plasma and secreted by circulating EPCs were measured before and after exercise.
RESULTS AND CONCLUSION: Flow cytometry analysis showed that the number of circulating EPCs increased after exercise   (P < 0.05). The acetylated-LDL and lectin fluorescent staining method indicated that the cultured EPCs also increased after exercise (P < 0.05). The proliferative and migratory activities of cultured EPCs were higher after exercise compared with before exercise (P < 0.05), but the adhesive activity did not change. Acute exercise also significantly enhanced plasma NO levels in healthy subjects (P < 0.05). There was a significant linear regression relationship between the enhanced plasma NO level and increased number or activity of circulating EPCs in healthy subjects (P < 0.05). Exercise can increase the number and activity of circulating EPCs in healthy subjects, which are related to the enhanced NO production in healthy subjects.

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