Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1211-1214.doi: 10.3969/j.issn.1673-8225.2012.07.017

Previous Articles     Next Articles

Toxicological effect of 50 μmol/L p38 signal transduction pathway inhibitors SB203580 via subconjunctival injection on rat corneas

Zhang Hai-feng, Zhong Yan-yan, Huang Shu-xin, Lu Xiao-he   

  1. Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-12-09 Revised:2011-12-29 Online:2012-02-12 Published:2012-02-12
  • Contact: Lu Xiao-he, Professor, Chief physician, Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China luxh63@163.com
  • About author:Zhang Hai-feng☆, Studying for doctorate, Attending physician, Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China surgeonzhf@163.com
  • Supported by:

    Guangdong Science and Technology Plan Projects, No.2008B080703037

PDF

339

Abstract:

BACKGROUND: SB203580 which is one of the special inhibitors of p38 signal transduction has the anti-inflammatory ability and can inhibit the growth of tumor vessels. However, the toxicity and irritation of SB203580 may be the obstacle of the application on eyes.
OBJECTIVE: To study the toxicological effect of SB203580 with subconjunctival injection on rat corneas.
METHODS: Right eyes of SD rats were preformed with subconjunctival injection of 0.04 mL of 50 μmol/L SB203580 and saline in the same dose respectively, once per day for 7 days. At 7 days after injection, the corneal inflammation index was recorded and all the right corneas were harvested for histopathological and ultrastructural examination.  
RESULTS AND CONCLUSION: Reversible anemia-like conjunctiva was observed in SB203580 group and disappeared at the second day after injection; After injection with SB203580 and saline, no significant difference of corneal inflammation index was found between the two groups (P > 0.05), but the ciliary congestion score in saline group was higher than that in SB203580 group (P < 0.05). Histological examination revealed that all rat corneal epithelium were completed, the stroma was arranged in order and the cortex was continuous. No obvious abnormal corneal structures and organelles were found under transmission electron microscope. This suggests that subconjunctival injection of 0.04 mL of 50 μmol/L SB2036580 had no obvious toxicological effect on rat corneas.

CLC Number: