Abstract:

BACKGROUND: Previous studies of our research group have indicated that both washed platelet and platelet-rich plasma promote the proliferation of human dental pulp cells in some concentration range.
OBJECTIVE: To further investigate the effect of washed platelet and platelet-rich plasma at different concentrations on the mineralization of human dental pulp cells.
METHODS: Human dental pulp cells from the healthy extracted teeth donated by patients under orthodontic treatment were cultured and passaged for 4-6 passages. Platelet-rich plasma and washed platelet were manufactured from venous blood of the same donor. The activity of alkaline phosphatase in dental pulp cells was determined using Alkaline Phosphatase Reagent Kit and Protein Quantification Reagent Kit on the 7th day after mineralization induction. The formation of mineralized nodules in dental pulp cells were observed by alizarin red staining on the 10th and 20th days after mineralization induction.
RESULTS AND CONCLUSION: Both washed platelet and platelet-rich plasma at the concentration of 10%-30% evidently promoted the alkaline phosphatase activity in dental pulp cells. The most effective concentration to promote the alkaline phosphatase activity was 20%. Both washed platelet and platelet-rich plasma at the concentration of 10%-30% evidently promoted the formation of mineralized nodules in dental pulp cells on the 10th day after mineralization induction. The mineralized nodules in the 10% concentration group were the largest on the 20th day after mineralization induction. There was no difference between the washed platelet and platelet-rich plasma at the same concentration.