Abstract:

BACKGROUND: The common method to culture the primary synovial cells is tissue cultivation, enzyme digestion and mechanical dispersion method.
OBJECTIVE: With four different methods to handle big rat synovial organization in vitro, and to observe the growth situations of synovial cells and explore the optimal method of knee joint synovial cells culture in vitro.
METHODS: The organize block groups and the shear ground groups were treated with four different approaches: ①Digested without enzyme digestion; ②Digested by using collagenase type Ⅱ; ③Digested by using trypsin; ④Digested sequentially by using collagenase and trypsin. After treatment, groups were cultured according to natural link wall and artificially stick wall modes, respectively. Growth situation of synovial cells were observed by using inverted microscope.
RESUTLS AND CONCLUSION: Nine days later, the number of cells in organization shear group treated without enzyme digestion and artificially stick wall was 2.03×106, and that was the largest number in all the culture method. The survival rate was the highest which could reach to 95%, which was better than natural link wall. The shape of the tissue, concentration of the enzyme and the way to link played an important role on cells culture. The optimal method to in vitro culture rats knee synovial cells was tissue shear ground without enzymes dealing and cultivated with artificially stick wall.