Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1173-1179.doi: 10.3969/j.issn.1673-8225.2012.07.009

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The mRNA alteration and correlation of Calpains and FoxOs during gastrocnemius muscle atrophy induced by sciatic nerve injury and cardiotoxin injection  

Wu Rong-hua, Xu Man, Zhao Pan-feng, Liu Mei   

  1. Key Laboratory of Neuroregeneration, Nantong University, Nantong  226001, Jiangsu Province, China
  • Received:2011-12-09 Revised:2012-01-04 Online:2012-02-12 Published:2012-02-12
  • Contact: Mei, Ph.D., Professor, Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China liumei@ntu.edu.cn
  • About author:Wu Rong-hua★, Master, Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China wuronghua5@126.com Xu Man★, Studying for master’s degree, Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China xuman2011@gmail.com
  • Supported by:

    Research Program of Education Ministry, No.208044*; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)*; Project of Innovation in Postgraduate of Nantong University, No.YKC09050*

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Abstract:

BACKGROUND: The calpain family members (Calpains) and FoxO transcription factors (FoxOs) play important roles during muscle atrophy.
OBJECTIVE: To investigate the different expression patterns and correlations of Calpains (Calpain 1, Calpain 2, Calpain 3) and FoxO transcription factors (FoxO1 and FoxO3a) during gastrocnemius muscle atrophy induced by sciatic nerve injury and cardiotoxin injection.
METHODS: The gastrocnemius muscle atrophic models were established by sciatic nerve injury and cardiotoxin injection. The mRNA level of Calpains and FoxOs were detected by real time quantitative PCR at 3, 7, 14 and 21 days after injury. The linear regression analysis was performed to conduct the correlations between Calpains and FoxOs.
RESULTS AND CONCLUSION: The expression level of Calpain 1 (CAPN1), Calpain 2 (CAPN2) and FoxO3a mRNA reached to peak at 7 days after sciatic nerve injury, and then decreased gradually; the mRNA level of Calpain 3 (CAPN3) and FoxO1 reached to peak at 14 days after sciatic nerve injury, then declined. After cardiotoxin injection, the expression level of CAPN1, CAPN2 and FoxO3a mRNA rapidly reached to peak at 3 days after cardiotoxin injection and then slowly went back down; the mRNA level of FoxO1 found its climax at 7 days after cardiotoxin injection, while that of CAPN3 instantly got to its lowest point at 3 days after cardiotoxin injection. The result of linear regression analysis showed that there was a significant positive correlation between the expression of CAPN3 and FoxO1 mRNA (r=0.901 5) during gastrocnemius muscle atrophy induced by sciatic nerve injury; And after cardiotoxin injection, the strong positive correlation existed between the mRNA level of CAPN1, CAPN2 and that of FoxO3a (r=0.898 7, r=0.937 4) respectively. The present study indicated that the mRNAs alteration of CAPN1 and CAPN2 was similar in the two different muscle injury models, while CANP3 displayed a different expression pattern. 

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