Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1169-1172.doi: 10.3969/j.issn.1673-8225.2012.07.008

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Effects of nerve growth factor injection via different ways on repair and regeneration of the sciatic nerve after its anastomosis

Wang Li-ming, Tang Ji-cun, Xin Lin-wei, Li Qiang, Zhou Ying-qiong, Chen Hao, Gu Zhen-lin   

  1. Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin  540001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-11-09 Revised:2011-12-08 Online:2012-02-12 Published:2012-02-12
  • Contact: Tang Ji-cun, Associate professor, Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin 540001, Guangxi Zhuang Autonomous Region, China tangjicun@126.com
  • About author:Wang Li-ming★, Studying for master’s degree, Physician, Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin 540001, Guangxi Zhuang Autonomous Region, China lmwang453@163.com
  • Supported by:

     Health Program of Guangxi Zhuang Autonomous Region, No. z2009046*

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Abstract:

BACKGROUND: Nerve growth factor (NGF) can promote the repair of nerve after nerve injury. However the advantages and disadvantages of different treatment methods are still unclear.
OBJECTIVE: To investigate the effects of intrathecal injection and local injection of NGF on repair and regeneration of rabbit sciatic nerve after its anastomosis.
METHODS: Sciatic nerves from 24 New Zealand white rabbits were cut-off and then sutured. NGF at 30 μg or saline at the same dose were injected intrathecally or locally into the rabbits.
RESULTS AND CONCLUSION: After 12 weeks of sciatic nerve injury, the sheath of sciatic nerve was thickened in the intrathecal injection group, nerve fibers arranged in neat rows and was similar with normal nerve fibers; the nerve conduction velocity, number of myelinated nerve fibers and myelin sheath thickness in the intrathecal injection group were obviously increased compared with the other groups (P < 0.05), the local injection group took the second place. NGF can promote the repair and regeneration of peripheral nerve after its anastomosis, and the intrathecal injection NGF is superior to local injection.

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