Abstract:

BACKGROUND: Diacerein is a novel interleukin-1 blockers, researches regarding whether it can inhibit the apoptosis of chondrocytes and whether it plays the role through signaling pathway or not are rately.
OBJECTIVE: To investigate the effects of Diacerein on the apoptosis of chondroctyes induced by interleukin-1 beta (IL-1β).
METHODS: Articular chondrocytes of Sprague Dawley rats were cultured in vitro and identified by hematoxylin-eosin staining and immunofluorescence staining of collagen typeⅡ. The rats apoptosis model of articular chondrocytes was induced by IL-1β, and then treated with different concentrations of diacerein (10-4, 10-5, 10-6 mol/L).
RESULTS AND CONCLUSION: Diacerein with the concentrations of 10-4 mol/L and 10-5 mol/L could decrease the apoptotic rate of chondrocytes which induced by IL-1β (P < 0.01, P < 0.05). Diacerein with the concentrations of 10-5 mol/L could inhibit phosphorylation-p38 which induced by IL-1β (P < 0.01). Compared with the control group, the expression of p38 mRNA of model group was decreased by 3.78 times (P < 0.01). Diacerein can inhibit IL-1β-induced early apoptosis. The possible mechanism is related to the effects of Diacerein on the inhibition of the expression the protein and gene of p38.