Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (7): 1157-1160.doi: 10.3969/j.issn.1673-8225.2012.07.005

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Basic fibroblast growth factors antagonize hydrogen peroxide damage to chondrocytes  

华中科技大学同济医学院附属同济医院骨科，湖北省武汉市   430030   

  1. Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430030, Hubei Province, China
  • Received:2011-09-07 Revised:2011-11-02 Online:2012-02-12 Published:2012-02-12
  • Contact: You Hong-bo, Doctor, Associate professor, Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China youhongbo003@yahoo.com.cn
  • About author:Zhao Wen-bin★, Studying for master’s degree, Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China tjwb525@hotmail.com

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Abstract:

BACKGROUND: Whether basic fibroblast growth factor (bFGF) can antagonize hydrogen peroxide damage to chondrocytes is still unclear.
OBJECTIVE: To observe the antagonism effect of bFGF on the hydrogen peroxide-induced cell damage to rat knee-joint chondrocytes.
METHODS: Chondrocyte apoptosis of Sprague Dawley (SD) rat knee-joint was induced by 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 and    3.2 mmol/L hydrogen peroxide. Effective concentration for induced apoptosis was chosen, and then 1 μg/L bFGF was added into it for intervention.
RESULTS AND CONCLUSION: The MTT data indicated that 0.2-1.6 mmol/L hydrogen peroxide could significantly induce apoptosis of chondrocytes of SD rat knee-joint. Apoptosis was increasing as the concentration was increased. The Hoechst33342 staining and flow cytometry results showed that 1 μg/L bFGF could antagonise the hydrogen peroxide-induced apoptosis to rat knee-joint chondrocytes.

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