Abstract:

BACKGROUND: It is difficult to culture osteoblasts, and different in cell number, purity, proliferation and differentiation activity obtained by different culture methods.
OBJECTIVE: To investigate the ideal culturing method of the human osteoblasts in vitro. 
METHODS: Human osteoblasts were isolated from trabecular bone of ilium by bone tissue and enzymatic digestion methods.
RESULTS AND CONCLUSION: The isolated and cultured human osteoblasts were in good condition and had a high purity. The cells were adherent growth, diversification in morphology, and mostly showed polygonal, spindle-shaped, triangular, and the cytoplasm was abundant and the spurt was stretched out. It has the typical characteristics of osteoblasts. The cells proliferation was well, alkaline phosphatase staining and mineralized nodules alizarin red staining was positive. We successfully cultured osteoblasts in vitro from trabecular bone of ilium by bone tissue and enzymatic digestion methods; it indicated that was the ideal method to culture osteoblasts.