Abstract:

BACKGROUND: Limb replantation success rate with limb preservation method is directly related to conduct in vitro simulated physiological environment foster limbs, which can extend saved time of the limbs.
OBJECTIVE: To investigate the effects of different oxygen concentrations on blood oxygen pressure in the system of in vitro physiological environment foster limbs.
METHODS: Totally nine New Zealand sheep with a total of 36 limbs underwent in vitro limb perfusion experiments with blood and were randomly divided into six groups according to different concentrations of oxygen gas (100%, 60%, 50%, 40%, 20%, 10%) into the membrane lung.
RESULTS AND CONCLUSION: Comparison of blood oxygen pressure in each group, the arterial oxygen values in experimental group 6 was in the normal range (80-100 mm Hg), and with the oxygen concentration decreases, arterial oxygen pressure values decreases. It is indicated that in vitro simulation of physiological environment in the foster care system, different concentrations of oxygen gas into the membrane lung may have an impact on the arterial partial pressure of oxygen; through reducing the oxygen concentration, the arterial oxygen pressure can be close to the normal range.