Abstract:

BACKGROUND: Studies find that ischemic preconditioning and ischemic postconditioning both have obvious protective effects on the ischemia-reperfusion-induced lung injury.
OBJECTIVE: To explore the cumulative protection effects of ischemic preconditioning combined with ischemic postconditioning on the ischemia reperfusion injury during lung transplantation.
METHODS: A total of 40 SD rats were randomly divided into sham operation group, model group, ischemic preconditioning group, ischemic postconditioning group and combined ischemic preconditioning and ischemic postconditioning group. Lung ischemic reperfusion injury model in rats was constructed in the latter four groups. The left hilus pulmonis in rats of the ischemia preconditioning group, ischemic postconditioning group and combined ischemic preconditioning and ischemic postconditioning group was blocked and opened for three circles before modeling or/and after modeling.
RESULTS AND CONCLUSION: Dry weight ratio, the acitivity of myeloperoxidase and the level of malondialdehyde in rat lungs of the combined ischemic preconditioning and ischemic postconditioning group were markedly lower than those in the ischemic preconditioning group and ischemic postconditioning group (P < 0.05); while the activity of superoxide dismutase was significantly higher in the combined ischemic preconditioning and ischemic postconditioning group than those in the ischemic preconditioning group and ischemic postconditioning group (P < 0.05); the pathological injury of the lungs reduced significantly. The activity of superoxide dismutase, the level of malondialdehyde and the activity of myeloperoxidase in rat lungs of the ischemic preconditioning group and ischemic postconditioning group were close to each other (P > 0.05); and the pathological injury of the lungs were close to each other. The levels of the superoxide dismutase and malondialdehyde in the ischemic preconditioning group and combined ischemic preconditioning and ischemic postconditioning group were positively correlated with myeloperoxidase level. These findings indicate that ischemic preconditioning combined with ischemic postconditioning have an obvious cumulative protection effect on lung tissue injury induced by the neutrophil infiltration, activation and oxidation, therefore it can further reduce the lung injury after ischemia reperfusion.