Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 775-778.doi: 10.3969/j.issn.1673-8225.2012.05.004

Previous Articles     Next Articles

Engraftment of human amniotic epithelial cells injected by different routes in the liver 

Zhang Hai-yan1, 2, Piao Zheng-fu1, 2, Ding Shu-qin2, Ren Feng1, 2, Isao Kamo3○, Norio Sakuragawa3○, Li Ning1, 2   

  1. 1Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China;      2Beijing Institute of Liver Disease, Beijing 100069, China; 3Department of Regenerative Medicine, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan
  • Received:2011-07-14 Revised:2011-09-18 Online:2012-01-29 Published:2012-01-29
  • Contact: Li Ning, Chief physician, Professor, Doctoral supervisor, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China
  • About author:Zhang Hai-yan★, Master, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China cellzhy@yahoo.com.cn Piao Zheng-fu★, Doctor, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China zfpiao88@yahoo.com.cn
  • Supported by:

    Science and Technology Development (General Program) of Education Committee of Beijing, No. KM200810025009; Science and Technology Activities Foundation for Selected Returned Overseas Chinese Scholars, No. 20080015 

PDF

379

Abstract:

BACKGROUND: Cell transplantation has a certain effect for liver disease, and it has its own advantages compared with the liver transplantation, but there are many issues to be resolved.
OBJECTIVE: To investigate the engraftment of human amniotic epithelial cells (hAECs) injected by different routes in liver of rats.
METHODS: hAECs were separated from placenta and labeled by PKH26 fluorochrome. hAECs (1×107 cells) were transplanted into SD rats through enterocoelia, portal vein, caudal vein or injected directly into the liver. Liver frozen sections were prepared after 3 days. The distribution of hAECs in liver was detected by converted fluorescence microscope. And survival condition of hAECs injected through portal vein in liver was observed by time dependent.
RESULTS AND CONCLUSION: hAECs were successfully transplanted into SD rat liver injected by portal vein, caudal vein and the route of liver injection, but hAECs injected intraperitoneally were not found in the liver. The hAECs that injected by portal vein could live for at least 16 days. Excessive cell injection causes the vascular thrombosis in liver and leading to necrosis. hAECs can live 16 days above without transplantation rejection. It indicates that amnion source cells may become one of the biomaterials for the treatment of liver diseases.

CLC Number: