Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 413-416.doi: 10.3969/j.issn.1673-8225.2012.03.007

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Preparation and biological characterization of c(RGDyK)@SiO2@Fe3O4 nanoparticles targeted for angiogenesis  

Sun Peng-fei1, Cao Xiang-rong1, Zhu Hao1, Jiao Zong-xian2, Zhang Feng-wei3, Xie Ke-rong1   

  1. 1Lanzhou University Second Hospital, Lanzhou  730030, Gansu Province, China; 2College of Basic Medical Science, Lanzhou University, Lanzhou  730000, Gansu Province, China; 3College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou 730000, Gansu Province, China
  • Received:2011-10-31 Revised:2011-11-08 Online:2012-01-15 Published:2012-01-15
  • About author:Sun Peng-fei, Associate chief physician, Associate professor, Master’s supervisor, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China sunpengfeiyy@ msn.com
  • Supported by:

    the Fundamental Research Funds for the Central Universities, No.lzujbky-2009-154*

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Abstract:

BACKGROUND: SiO2 has a great amount of hydroxyl groups, It can be further functionalized to couple targeting ligand in order to expand the application of Fe3O4@SiO2 nanoparticles in the biomedical field.
OBJECTIVE: To investigate the preparation method of targeted c(RGDyK)@SiO2@Fe3O4 nanoparticles (NPs) and its property testing.
METHODS: Oleic acid-loaded hydrophobic Fe3O4 NPs were prepared via a pot chemical coprecipitation, and the biocompatible Fe3O4@SiO2 compound NPs were synthesized with a reverse microemulsion. By means of the 3-aminopropyl triethoxysilan (APTMS) as couplant, the hydroxyl groups of SiO2 surface in compound particles were changed into amino or aldehyde groups. Then 1.0 mg of c(RGDyK) peptide was added into the reaction system under ultrasound shake in order to prepare c(RGDyK)@SiO2@ Fe3O4 NPs. The Fe3O4@SiO2 or c(RGDyK)@SiO2@Fe3O4 NPs were co-cultured with EA.hy926 cells for detection at 24, 48 and 72 h hours.
RESULTS AND CONCLUSION: Fe3O4@SiO2 NPs prepared by a pot chemical coprecipitation and reverse microemulsion had a mean diameter of 40 nm, and c(RGDyK) peptide was successfully connected to SiO2 surface of compound NPs via APTMS. After EA.hy926 cells were cultured accompanied with Fe3O4@SiO2 or c(RGDyK)@SiO2@Fe3O4 NPs for 24 hours, the cell activity was significantly improved (P < 0.05), especially with the c(RGDyK)@SiO2@Fe3O4 NPs, and there was no difference in cell activity between the control group and c(RGDyK)@SiO2@Fe3O4 group at 72 hours (P > 0.05). Electron microscopy showed that the ability of EA. hy926 cells phagocytizing targeted c(RGDyK)@SiO2@Fe3O4 NPs was more stronger than no-targeted Fe3O4@SiO2 NPs at 24 hours of cell culture. The c(RGDyK)@SiO2@Fe3O4 NPs are a kind of excellent biomaterial because of their biocompatibility, superparamagnetism and higher ability of targeted for vascular endothelial cells.

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