Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 391-395.doi: 10.3969/j.issn.1673-8225.2012.03.002

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Preparation and characterization of poly(hydroxybutyrate-co-hydroxyvalerate) nanofiber scaffolds using thermally induced phase separation method

Chen Cong, Wang Ying-jun, Shao Jun-dong, Du Chang   

  1. State Research Center of Human Functional Reconstruction Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou  510641, Guangdong Province, China
  • Received:2011-05-16 Revised:2011-06-06 Online:2012-01-15 Published:2012-01-15
  • Contact: Wang Ying-jun, Doctor, Professor, State Research Center of Human Functional Reconstruction Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China imwangyj@163.com
  • About author:Chen Cong★, Studying for master’s degree, State Research Center of Human Functional Reconstruction Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China cong.happy02@ gmail.com
  • Supported by:

    Program for Changjiang Scholars and Innovative Research Team in University, No. IRT0919*; Central University Basic Research Fund of South China University, No. 2009zz0004*; New Century Excellent Talents Funding, No. NCET-08-0210*

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Abstract:

BACKGROUND: Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) is a polymer of microbial source with excellent biocompatibility and processing performance, which has great potential to be used as a biological material.
OBJECTIVE: To investigate the synthesis methods and crystallization of nanofibrous matrix of a semicrystalline biopolyester PHBV via thermally induced phase separation.
METHODS: The formation of the matrix was characterized by scanning electron microscopy, wide angle X-ray diffraction, Fourier transform infrared and differential scanning calorimetry.
RESULTS AND CONCLUSION: The quenching/gelation temperatures showed a great effect on the crystallization and thermal property of the nanofibers, then affected the morphology of the scaffolds. The crystallization of the PHBV matrix during the phase separation process primarily involved HB units with small amounts of crystals incorporating HV units at higher gelation temperatures. When the quenching/gelation temperature decreased, the crystallinity and crystallite size were decreased but better crystalline perfection was obtained by excluding HV units from the PHB lattice. Meanwhile, cells experimental results show that nanofibers structure of scaffolds are really more conducive to cell adhesion and growth. The morphological and structural dependence on temperature may provide a simple way to fine tune the properties of PHBV scaffolds including biodegradability and bioresponsiveness to cellular activity.

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