Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 320-324.doi: 10.3969/j.issn.1673-8225.2012.02.030

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Induction of heme oxygenas 1 by sulforaphane of INS-1 cells

Yang Yi1, 2, Yu Zhi-wen2, 3, Shao Wei-juan2, Jin Tian-ru2   

  1. 1Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China; 2Banting and Best Diabetes Centre, University of Toronto, Toronto  M5G 1L7, Ontario, Canada; 3Key Laboratory of Food Nutrition and Health, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-10-08 Revised:2011-11-12 Online:2012-01-08 Published:2012-01-08
  • Contact: Jin Tian-ru, Doctor, Associate professor, Banting and Best Diabetes Centre, University of Toronto, Toronto M5G1L7, Ontario, Canada tianru.jin@utoronto.ca
  • About author:Yang Yi★, Master, Associate professor, Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Banting and Best Diabetes Centre, University of Toronto, Toronto M5G1L7, Ontario, Canada yangyi73422@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81160103*; the Ningxia Hui Autonomous Region Technology Research International Technology Cooperation Plan, No. NXIC2011010*; Natural Science Foundation of Ningxia, No. NZ1097*; the Startup Project in Research for Special Talents of Ningxia Medical University, No. XT20*

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Abstract:

BACKGROUND: Sulforaphane (SFN) can be used for oxidative stress related disease’s treatment. Heme oxygenas1 (HO-1) is a kind of enzyme degrading stress hemoglobin protein and HO-1 has become the first choice of anti-oxidation process.
OBJECTIVE: To study the effect of SFN induced expression of HO-1 in a rat pancreatic beta cell line INS-1 and the potential cytoprotective mechanism of nuclear factor erythroid-related factor 2 (Nrf2).
METHODS: During the culture of INS-1 cells, 3 μmol/L SFN was incubated for 3 hours and then glucose oxidase, dexamethasone and glucosamine were added to establish insulin resistance models.
RESULTS AND CONCLUSION: The expression of HO-1 protein in INS-1 cells was increased after SFN (3μM) exposure, and reached the highest level after 4 hours exposure (P < 0.05). Decreased expression of HO-1 protein which was induced by inducers of insulin resistance could be reversed by SFN (3 μM) (P < 1 005). A positive correlation was found between the expression of HO-1 protein improved by SFN and the improved expression of protein kinase B phosphorylation in INS-1 cells treated with glucosamine (P < 0.05, r=0.23). SFN may enhance antioxidative defense in pancreatic beta cells through the induction of HO-1 via Nrf2 mediated transcription and enhance insulin signaling to protect beta cells from damaging.

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