Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 299-302.doi: 10.3969/j.issn.1673-8225.2012.02.025

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Role of transient receptor potential melastatin 7 in rat RBL-2H3 cells survival and apoptosis  

NG Ngai-Mui1, Jiang Shan-ping2, Huang Lin-jie2   

  1. 1Department of Clinical Medicine, School of Medicine, Shenzhen University, Shenzhen  518060, Guangdong Province, China; 2Department of Respiratory Medicine, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou  510120, Guangdong Province, China
  • Received:2011-10-11 Revised:2011-11-09 Online:2012-01-08 Published:2012-01-08
  • Contact: Jiang Shan-ping, Chief physician, Professor, Doctoral supervisor, Department of Respiratory Medicine, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong Province, Chinashanpingjiang @126.com
  • About author:NG Ngai-Mui☆，Doctor, Lecturer, Department of Clinical Medicine, School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, China gzmeggiewoo@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 81070027*; the Natural Science Foundation of Guangdong Province, No. 10151008901000078*

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Abstract:

BACKGROUND: Transient receptor potential melastatin 7 is an important calcium channel in mast cells. However, the role of transient receptor potential melastatin 7 in mast cell survival and apoptosis is unclear.
OBJECTIVE: To investigate the role of transient receptor potential melastatin 7 in rat RBL-2H3 cells survival and apoptosis.
METHODS: RBL-2H3 cells in logarithmic growth phase were intervened with 50, 100 and 200 μmol/L transient receptor potential channel blocker 2-APB. Cells intervened with 0.1% DMSO and normally cultured cells were used as control. RBL-2H3 cells were transfected with retrovirus vector of transient receptor potential melastatin 7-siRNA. Empty vector group and control group were constructed.
RESULTS AND CONCLUSION: At 72 hour after treated with 100 and 200 μmol/L 2-APB, RBL-2H3 cell number was decreased. The optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). At 72 hour after RBL-2H3 cells were transfected with transient receptor potential melastatin 7-siRNA, the optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). These findings indicate that transient receptor potential melastatin 7 channel involve in the process of survival and apoptosis in RBL-2H3 cells.

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