Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 295-298.doi: 10.3969/j.issn.1673-8225.2012.02.024

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Apoptosis of anterior horn motor neurons after hyperbaric oxygen preconditioning in spinal cord injury rats

Li Hong-peng1, Ba Fang2, Bai Dan1, Gao Jie1   

  1. 1Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang  110001, Liaoning Province, China;
    2Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang 110006, Liaoning Province, China
  • Received:2011-10-19 Revised:2011-11-10 Online:2012-01-08 Published:2012-01-08
  • Contact: Li Hong-peng, Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China lihongpeng1966@hotmail.com
  • About author:第一作者及通讯作者：李洪鹏☆，男，1966年生，辽宁省海城市人，博士，副教授，主要从事脊髓损伤与修复研究。 lihongpeng1966@hotmail.com 共同第一作者：巴方，女，1975年生，辽宁省沈阳市人，在读博士，讲师，主要从事脊髓损伤与修复研究。baf126@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872669*
       

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Abstract:

BACKGROUND: Spinal cord injury is difficult to repair. The protection of relict neurons is the key for promoting neural regeneration.
OBJECTIVE: To test whether spinal cord anterior horn motor neurons are protected by hyperbaric oxygen preconditioning through inhibition of early apoptosis.
METHODS: A total of 26 Wistar male rats were randomly divided into two groups: control group and hyperbaric oxygen group. Rats in hyperbaric oxygen group were administrated with hyperbaric oxygen for 5 days. Complete spinal cord (T9-T10) transection model was constructed on the 5th day after hyperbaric oxygen. In the meantime, complete spinal cord (T9-T10) transection model was constructed for control rats.
RESULTS AND CONCLUSION: Nissl staining demonstrated that hyperchromic cells were common in the two groups at 8 hour and 1 day after spinal cord transection at T9–T10. The number of hyperchromic cells in the hyperbaric oxygen group was less than that in the control group. TUNEL staining demonstrated that the massive apoptotic neurons were found at 8 hour and 1 day after the spinal cord transection at T9–T10 in rat anterior horn of spinal cord in both groups; the number of apoptotic neurons decreased on the 3rd day. The number of apoptotic spinal cord anterior horn motor neurons in the hyperbaric oxygen group was smaller than that in the control group at 8 hour and 1 day after hyperbaric oxygen (P < 0.05,   P < 0.01). These findings indicate that hyperbaric oxygen preconditioning has a protective effect on anterior horn motor neurons after spinal cord injury.

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