Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 273-276.doi: 10.3969/j.issn.1673-8225.2012.02.019

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Viability of tissue fragments in colon and rectum detected using modified Alamar Blue assay  

Gan Jia-liang1, Gao Feng1, Cao Yun-fei1, Liao Cun2, Huang Jia-hao2, Tang Shuang-yi3   

  1. 1Department of Colorectal and Anal Surgery,  3Department of Pharmacy, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China;
    2Postgraduate College of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-01 Revised:2011-12-02 Online:2012-01-08 Published:2012-01-08
  • Contact: Gao Feng, Doctor, Professor, Department of Colorectal and Anal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gaofeng0771@126.com
  • About author:Gan Jia-liang☆, Studying for doctorate, Department of Colorectal and Anal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gjl259@126.com
  • Supported by:

    the Scientific Research Foundation of Education Bureau of Guangxi Zhuang Autonomous Region, No. 201012MS039*, 201012MS041*; Innovation Project of Guangxi Graduate Education No.2011105981002D34*

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Abstract:

BACKGROUND: Alamar Blue assay is one of the common methods to evaluate histic or cellular viability. It is widely used to detect cell viability at present. However, using Alamar Blue assay to detect the viability of tissue fragments has not been reported.
OBJECTIVE: To detect the viability of the regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum using modified Alamar Blue assay.
METHODS: Based on cell viability detect assay of Alamar Blue, the viability of the regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum was assessed using absorbance per unit mass. The detecting time was chosen at 0, 6, 12, 18, 24 , 30 and 36 hours according to the specification of the Alamar Blue assay and the pre-experiment results. Every specimen was divided into seven groups and the blank control hole was established.
RESULTS AND CONCLUSIONS: Absorbance per unit mass reflected the change tendency of proliferation activity in tissue fragments as the culture time went on. Both regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum reached the best proliferative state at 12-24 hours after culture. It confirms that the modified Alamar Blue assay is a sensitive and effective way to evaluate the viability of colorectal tissue fragments.

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