Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 107-110.doi: 10.3969/j.issn.1673-8225.2012.01.023

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Effects of Chinese herbal compound on acute myeloid leukemia stem cells

Liu Kui, Xu Rui-rong, Hu Shu-bo, Wang Xiao-ling, Cui Xing, Wang Yan, Wang Jing-yi   

  1. Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan  250011, Shandong Province, China
  • Received:2011-06-12 Revised:2011-07-09 Online:2012-01-01 Published:2012-01-01
  • About author:Liu Kui★, Master, Physician, Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Shandong Province, China mhfxq@163.com
  • Supported by:
    the National Natural Science Foundation of China, No. 30672746*
     

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Abstract:

BACKGROUND: The existence of leukemic stem cells (LSCs) in leukemic patients is the basment how leukemia occurs and develops, drug resistance and recurrence happens.
OBJECTIVE: To investigate the effects of Chinese herbal compound on the LSCs of acute myeloid leukemia (AML) patients.
METHODS: CD34+CD38- LSCs were isolated from the bone marrow of 30 AML patients by immunomagnetic beads. The cells were divided into two groups: Control group were treated without medicine, while experiment group were treated by Chinese herbal compound preparation with terminal concentration of 100 mg/L.
RESULTS AND CONCLUSION: The inhibition rate of Chinese herbal compound to LSCs was (36.660±0.018)%. Nuclear factor κB (NF-κB) expressed in 22 specimens, and the positive rate was 73.33% (22/30). The activity of NF-κB was tested by electrophoretic mobility. DNA-protein band grey values were analyzed by gel imaging system.The activity of NF-κB in the experiment group was decreased compared with that in the control group (P < 0.05) by comparison of the DNA-protein band grey values. The results showed that Chinese herbal compound may interfere in the growth of LSCs by inhibiting the activity of NF-κB.
 

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