Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 81-84.doi: 10.3969/j.issn.1673-8225.2012.01.017

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Differentiation of neural stem cells into glial cells cultured in serum-free medium containing growth factors by using neurosphere assay

Huang Fei, Zhao Ji-tong, Shen Qiang   

  1. 上海交通大学附属上海市第一人民医院骨科， 上海市  200080
  • Received:2011-07-17 Revised:2011-08-15 Online:2012-01-01 Published:2012-01-01
  • Contact: Shen Qiang, Doctor, Chief physician, Professor, Master's supervisor, Department of Orthopedics, First People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200080, China shmail1231@yahoo.com
  • About author:Huang Fei★, Studying for master’s degree, Department of Orthopedics, First People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200080, China huangfei3738@126.com

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Abstract:

BACKGROUND: Isolation and culture of neural stem cells in vitro and to master their biological characteristics are the basis of neural stem cells transplantation.
OBJECTIVE: To isolate and culture cerebral cortex neural stem cells from rat embryos by neurosphere assay, and to observe the growth and differentiation characteristics of neural stem cells.
METHODS: Neural stem cells were isolated from rat embryonic cerebral cortex and cultured in serum-free medium. Immunocytochemical staining was used to examine neural stem cells and differentiated cells markers.
RESULTS AND CONCLUSION: Neural stem cells grew as free-floating neurospheres in the serum-free conditions, but spontaneous fusion occurred among some neurospheres. Immunocytochemical staining showed these neruospheres positively expressed nestin, and neural stem cells could differentiated into neurons, astrocytes and oligondendrocytes, but mainly differentiated into astrocytes. In serum-free medium containing growth factors, cerebral cortex neural stem cells could be isolated and cultured by using neurosphere assay.

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