Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9999-10002.doi: 10.3969/j.issn.1673-8225.2011.53.029

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Effect of exogenous antigen on expression of major histocompatibility complex class I chain-related gene A in endothelial cells

Wang Yun-yan1, Hou Jian-quan2, He Jun3, Yuan Xiao-ni3, Zhang Jiang-lei2, Wen Duan-gai2   

  1. 1Department of Urinary Surgery, First Affiliated Huaian Hospital of Nanjing Medical University, Huaian  223300, Jiangsu Province, China
    2Department of Urinary Surgery, First Affiliated Hospital of Soochow University, Suzhou  215006, Jiangsu Province, China
    3Haematology Research Institute of Jiangsu Province, First Affiliated Hospital of Soochow University, Suzhou  215006, Jiangsu Province, China
  • Received:2011-06-11 Revised:2011-08-07 Online:2011-12-31 Published:2011-12-31
  • Contact: Hou Jian-quan, Doctor, Professor, Chief physician, Department of Urinary Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • About author:Wang Yun-yan☆, Doctor, Department of Urinary Surgery, First Affiliated Huaian Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, China wangyunyan2000@hotmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872530*; the Natural Science Foundation of Jiangsu Province, No. BK2007056*; the Key Talents Project of Health Department of Jiangsu Province, No. RC2007079*

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Abstract:

BACKGROUND: Studies have demonstrated that incidence rate of acute rejection in renal transplant recipients with pre-production of major histocompatibility complex class I chain-related gene A (MICA), including parts of autoantibody, before transplantation in body, is obviously greater than that of recipients with negative antibody. 
OBJECTIVE: To investigate effects of exogenous antigen on MICA expression in endothelial cells.
METHODS: The endothelial cells were cultured with exogenous recombinant MICA protein (group M5, M10 and M25) and heat shock protein-70 (group H5, H10 and H25) with dosages of 5, 10 and 25 μg/L, respectively, for 48 hours. Same volume of phosphate buffer saline was added into the control groups. 
RESULTS AND CONCLUSION: At 48 hours after induction, the expressions of MICA mRNA and protein were increased significantly in each experimental group (M5, M10 and M25) than that of the control group with significant (P < 0.05). The expression of MICA mRNA and MICA protein of group M5 and group M10 were remarkably higher than group M25 (P < 0.05); however, there was no significant difference between group M5 and M10 (P > 0.05). The expression of MICA membrane protein in the group M10 was obviously greater than that of the group M5 and M25 (P < 0.05). The level of soluble MICA (sMICA) in experimental groups (M5, M10 and M25) was decreased obviously comparing with that of the control group. These differences had statistical significances (P < 0.05). But there was no significant difference among the experimental groups (P > 0.05). However, the expression of MICA gene and sMICA level did not change after heat shock protein-70 stimulation. The exogenous MICA antigen up-regulates the expression of MICA mRNA and protein, especially increases the expression of membrane protein on the cell surface significantly, but sMICA in supernatant was dramatically decreased.

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