Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9215-9217.doi: 10.3969/j.issn.1673-8225.2011.49.022

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Effect of scopion venom component Ⅲ on expression of toll like receptor 4 and p65 in lipopolysaccharide stimulated human leukemia monocytic THP-1 cells

Song Xiang-feng1, 2, Niu Jie3, Liu Yu-feng1   

  1. 1Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou  453001, Henan Province, China; 2Department of Immunology, 3Laboratory of Morphology, Xinxiang Medical University, Xinxiang  453003, Henan Province, China
  • Received:2011-06-06 Revised:2011-10-10 Online:2011-12-03 Published:2011-12-03
  • About author:Song Xiang-feng★, Master, Associate professor, Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 453001, Henan Province, China; Department of Immunology, Xinxiang Medical University, Xinxiang 453003, Henan Province, China xfsong@xxmu.edu.cn
  • Supported by:

    Grants of Henan Education Bureau, No. 2010A31005*

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Abstract:

BACKGROUND: In recent years, studies have shown that toll-like receptors and nuclear factor κB (NF-κB) have a close relation with the occurrence and development of leukemia. However, there were no reports about the effect of scorpion venom on toll-like receptors on the surface of tumor cells and NF-κB.
OBJECTIVE: To investigate the effect of scopion venom component Ⅲ (SVC-Ⅲ) on expression of toll like receptor 4 (TLR4) and p65 in lipopolysaccharide (LPS) stimulated human leukemia monocytic THP-1 cells．
METHODS: THP1 cells were inoculated in 24-well cells culture plates, and there were five groups: control group, LPS (1 mg/L) stimulation group and SVC-Ⅲ treatment group (LPS+ SVC-Ⅲ, 1, 10, 20 mg/L). Real-time PCR was employed to detect the expression of TLR4 and p65 mRNA in THP1 cells，Western blot was used to detected the expression of TLR4 and p65 protein．
RESULTS AND CONCLUSION: The expression of TLR4 and p65 mRNA and protein of THP1 cells in LPS stimulation group was significantly higher than that in the control group (P < 0.01). However, the expression of TLR4 and p65 mRNA and protein of THP1 cells in SVC-Ⅲ treatment group was lower than that in LPS stimulation group (P < 0.01) and in dose-dependent manner. SVC-Ⅲ can down-regulate the expression of TLR4 and inhibit the activity of NF-κB in LPS stimulated THP1 cells in order to inhibit the cells proliferation．

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