Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9121-9127.doi: 10.3969/j.issn.1673-8225.2011.49.001

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Biological characteristics of bone marrow mesenchymal stem cells derived from coagulation factor Ⅸ knockout mice

Lü Min-min1, Lin Hong-gang1, Zhou Guang-qian1, 2   

  1. 1State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu  610041, Sichuan Province, China
    2Medical College of Shenzhen University Guangdong Province, China
  • Received:2011-05-16 Revised:2011-06-15 Online:2011-12-03 Published:2011-12-03
  • Contact: Zhou Guang-qian, Professor, Doctoral supervisor, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; Medical College of Shenzhen University Guangdong Province, China wormoscz@gmail.com
  • About author:Lü Min-min★, Studying for master’s degree, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China lvminmin2004@163.com
  • Supported by:

    the National 863 Program of China, No. 2007AA02Z119*

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Abstract:

BACKGROUND: Coagulation factor Ⅸ knockout may affect the biological characteristics of bone marrow mesenchymal stem cells (BMSCs) of the bone marrow microenvironment by affecting the hematopoietic system stability.
OBJECTIVE: To characterize the biological properties of BMSCs derived from coagulation factor Ⅸ knockout mice.
METHODS: BMSCs were isolated from coagulation factor Ⅸ knockout mice C57BL/6J-F9 tm1. Dws by using a modified adherent culture method. The cells were detected for their expression of general MSCs surface markers by using flow cytometry. The functionality was assessed for their multipotency to differentiate into osteoblasts, adipocytes and chondrocytes upon certain induction conditions.
RESULTS AND DISCUSSION: The BMSCs showed spindle-shape morphology and high capacity for proliferation and self-renewal. The cells were positive for CD44, CD56, CD73, CD90, CD105, CD106, CD166 and CD271, but negative for CD34 and CD11b. They demonstrated the ability to differentiate into osteoblasts, adipocytes and chondrocytes. These results illustrate the BMSCs derived from coagulation factor Ⅸ knockout mice C57BL / 6J-F9 tm1. Dws have perfect stem cell biological characteristics. The results initially suggest that deficiency of coagulation factor Ⅸ has no effect on the biological characteristics of BMSCs.

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