Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8661-8664.doi: 10.3969/j.issn.1673-8225.2011.46.026

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Cloning and sequencing of mature peptiede of human bone morphogenetic protein 4 cDNA

Yuan Shao-hui, Liu Wei, Wu Bin-qi, Han Xi-guang, Bi Zheng-gang   

  1. Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin   150001, Heilongjiang Province, China
  • Received:2011-05-06 Revised:2011-08-10 Online:2011-11-12 Published:2011-11-12
  • Contact: Bi Zheng-gang, Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China yuansh1970@ sina.com
  • About author:Yuan Shao-hui☆, Ph.D., Associate chief physician, Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China yuansh1970@ sina.com
  • Supported by:

    the Natural Science Foundation of Heilongjiang Province, No. D200876*; a grant from Scientific Technology Research Program of Education Department of Heilongjiang Province, No.11531149*

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Abstract:

BACKGROUND: Studies have shown that bone morphogenetic protein 4 (BMP-4) has strongest osteoinductive capacity, but its content in tissue is low.
OBJECTIVE: To clone human BMP-4 (hBMP-4) mature peptide gene. 
METHODS: According to the hBMP-4 sequence reported in Genebank, two primers were designed. The gene sequence of hBMP-4 mature peptide was gained by one step RT-PCR from the mRNA, which was extracted from human placenta. Then it was cloned into the vector of PGEM-T and the resultant plasmid was transformed into DH5α.The sequence of the plasmid BMP-4 was detected by DNA sequence machine. 
RESULTS AND CONCLUSION: DNA agarose electrophoresis showed that the product of RT-PCR was about 370 bp and it was consistent with the reported hBMP-4 sequence in Genebank. These findings suggest that the mature peptide of hBMP-4 gene can be successfully cloned from human placenta by RT-PCR.

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