Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8665-8667.doi: 10.3969/j.issn.1673-8225.2011.46.027

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Establishment of a method for primary culture of mouse Leydig cells in vitro

Zhao Wei-min1, Yang Jian-ying2, Dai Tao1, Zhang Gong-bao3, Yuan De-pin3   

  1. 1Luoyang Dongfang Hospital, Third Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Provice China
    2College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang  471003, Henan Provice  China
    3Department of Burn and Plastic Surgery, China Pingmei Shenma Medical Group General Hospital, Pingdingshan  467000, Henan Province China
  • Received:2011-04-18 Revised:2011-05-13 Online:2011-11-12 Published:2011-11-12
  • Contact: Yang Jian-ying, Doctor, Associate professor, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, Henan Province China jyyang@yahoo.cn
  • About author:Zhao Wei-min, Associate chief physician, Luoyang Dongfang Hospital, Third Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province China jyyang2010@163.com
  • Supported by:

    Science and Technology Development of Henan Province, No. 112102310144*; the Natural Science Research Development Program of Education Department of Henan Province, No. 2011B310004*; Ph.D. Programs of Henan University of Science and Technology, No. 09001285*

Abstract:

BACKGROUND: Tissue explant culture of Leydig cells leads to difficult control of contamitation and trypsin digestion of Leydig cells may injury cells.
OBJECTIVE: To establish a simple and effective method for primary culture of mouse Leydig cells.
METHODS: Mature mouse Leydig cells were digested by collagenase digestion and were purified by Percoll gradient centrifugation. Cell viability was identified by Trypan blau staining and cell purity was determined by 3 β-hydroxysteroid dehydrogenase staining.
RESULTS AND CONCLUSION: The cell purity was > 90%. The Leydig cells retained complete morphology, raid proliferation and good adherence. Results showed that an in vitro primary culture model of mouse Leydig cells was successfully established.

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