Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8542-8546.doi: 10.3969/j.issn.1673-8225.2011.45.041

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In vitro experiment of umbilical cord mesenchymal stem cells to promote bone healing and clinical application in one case

Li Hua-tao1, Bian Lin-fen1, Wu Sheng-gang1, Li Qiang1, Wang Li-li1, Dai Yu-cheng2, Liu Yang3   

  1. 1Burns unit, the 98 Hospital of Chinese PLA, Huzhou  313000, Zhejiang Province, China
    2Molecular Medicine Center of Second Affiliated Hospital of Nanchang University Medical School, Nanchang  330000, Jiangxi Province, China
    3Consonance Eastern China Company of Stem Cell Gene Engineering, Huzhou  313000, Zhejiang Province, China
  • Received:2011-08-03 Revised:2011-09-10 Online:2011-11-05 Published:2011-11-05
  • Contact: Dai Yu-cheng, Researcher, Molecular Medicine Center of Second Affiliated Hospital of Nanchang University Medical School, Nanchang 330000, Jiangxi Province, China
  • About author:Li Hua-tao☆, Doctor, Associate chief physician, Burns unit, the 98 Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China eehuatao@163.com

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Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells (UCMSCs) are more original than adult ones. Studies have shown that UCMSCs have higher telomerase activity, shorter culture doubling time and wider lineage differentiation.
OBJECTIVE: To explore the potency of UCMSCs differentiated into osteoblasts in vitro and their clinical effects on bone nonunion.
METHODS: UCMSCs were isolated from Wharton’s jelly of human umbiIical cord; after serial subculture in vitro, MSCs were tested for their immunophenotype and osteogenic differentiation. Clinical application of UCMSCs transplantation was also performed to a patient with refractory exposed bone fracture evoked by infection.
RESULTS AND CONCLUSION: UCMSCs exhibited fibroblastic morphology and long-term stable culture. These passaged cells presented MSCs immunophenotype and there were a great amount of calcium deposition and nodes in intracytoplasm detected by alizarin red and Von Kossa staining, respectively, after osteogenic differentiation. The patient was treated with UCMSCs suspension for four times, granulation tissue grew rapidly to fulfill the sinus tract and became epithelized. Twelve days after treatment, the fracture was healed. It indicates that UCMSCs have self-renewal and pluripotent differentiation potential and can differentiate into osteoblasts in vitro. For treatment of bone nonunion, UCMSCs has the ability to improve microenvironment.

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