Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8503-8507.doi: 10.3969/j.issn.1673-8225.2011.45.032

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Human umbilical cord mesenchymal stem cells induce ossification and treat bone defects

Qu Zhi-guo, Ye Xiang-yang, Lin Hui, Cui Zheng-hong   

  1. Department of Orthopedic Surgery, the Central Hospital of Siping City, Siping 136000, Jilin Province, China
  • Received:2011-04-10 Revised:2011-05-20 Online:2011-11-05 Published:2011-11-05
  • About author:Qu Zhi-guo, Chief physician, Department of Orthopedic Surgery, the Central Hospital of Siping City, Siping 136000, Jilin Province, China xyyegk@yahoo.cn Ye Xiang-yang☆, Doctor, Associate chief physician, Department of Orthopedic Surgery, the Central Hospital of Siping City, Siping 136000, Jilin Province, China xyyegk@yahoo.cn Qu Zhi-guo and Ye Xiang-yang contributed equally to this paper.

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Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells have greater expansion capacity and low immunogenicity in ossification and tissue and organ repair, and their growth potential into a colony is earlier than mesenchymal stem cells of the bone marrow and other sources.OBJECTIVE: To observe the clinical effect of umbilical cords mesenchymal stem cells on inducing ossification and treating bone defects.
METHODS: Human umbilical cord mesenchymal stem cells were isolated by the tissue block culture. In vitro osteogenic ability was confirmed by the morphology changes of human umbilical cord mesenchymal stem cells, alizarin red staining, alkaline phosphatase staining, and type Ⅰ collagen expression. Human umbilical cord mesenchymal stem cells transplantation was performed in patients with bone defects, and conditions of callus growth were regularly reviewed after transplantation.
RESULTS AND CONCLUSION: In vitro induction experiments confirmed that umbilical cord mesenchymal stem cells had a clear osteogenic role. Two months after cell transplantation, X-rays showed bony callus formation, fracture interspace vague. Osseous connection formation was found at 3 months after cell transplantation. It was confirmed that umbilical cord mesenchymal stem cells exerted a role of osteogenic induction, and could repair bone defects via in vivo cell transplantation.

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