Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8491-8494.doi: 10.3969/j.issn.1673-8225.2011.45.029

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Detection of Toll-like receptor 9 in peripheral blood mononuclear cells and serum tumor necrosis factor and Fas in patients with acute myeloid leukemia

Qiu Yuan-xiu, Mo Dong-hua, Wang Xiao-tao   

  1. Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-07 Revised:2011-06-11 Online:2011-11-05 Published:2011-11-05
  • Contact: Mo Dong-hua, Master’s supervisor, Associate professor, Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China tonyqyx@163.com
  • About author:Qiu Yuan-xiu★, Studying for master’s degree, Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China tonyqyx@163.com
  • Supported by:

    the Plan Program of Health Department of Guangxi Zhuang Autonomous Region, No.Z2010274, Z2011200**

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Abstract:

BACKGROUND: Toll-like receptor 9 (TLR9), tumor necrosis factor alpha (TNF-α) and Fas may together participate in the occurrence and development of acute myeloid leukemia (AML).
OBJECTIVE: To study the expression of TLR9 in peripheral blood mononuclear cells (PBMNCs), levels of TNF-α and apoptosis-related genes in serum of patients with acute myeloid leukemia (AML).
METHODS: The expression of TLR9 mRNA in PBMNCs of AML patients and normal control group was detected by reverse transcription-polymerase chain reaction (RT-PCR), the serum levels of TNF-α and Fas were detected were by enzyme-linked immunosorbent assay in each group.
RESULTS AND CONCLUSION: Compared to normal control group, the expression of TLR9 mRNA in PBMNCs of in AML patients with untreated and refractory/relapsed groups was significantly increased (P < 0.01), and complete remission group was not statistically significant (P > 0.05). The serum levels of TNF-α and Fas in AML patients were significantly higher than those in the normal control group (P > 0.01). The expression of TLR9 was positively correlated with the serum levels of TNF- α and Fas.

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