Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8487-8490.doi: 10.3969/j.issn.1673-8225.2011.45.028

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Immunophenotype of acute lymphoblastic leukemia and detection of CD4+CD25+ regulatory T cells

Wu Cui-ping1, Qin Xi1, Wu Cui-yun2, Zhu Hong1, Zhou Hai-yan1   

  1. 1Affiliated Hospital of Hainan Medical College, Haikou  570102, Hainan Province, China
    2Haikou People’s Hospital, Haikou  570208, Hainan Province, China
  • Received:2011-08-10 Revised:2011-09-07 Online:2011-11-05 Published:2011-11-05
  • Contact: Qin Xi, Professor, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China qinxi99@21cn.com
  • About author:Wu Cui-ping, Associate chief examiner, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China wucuiping2007@163.com
  • Supported by:

    the Science and Technology Research Project of Hainan Health Bureau, No. 2008-55*

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Abstract:

BACKGROUND: CD4+CD25+ regulatory T cells (Treg) may have a certain regulatory effect on immune response in the pathogenic process of acute lymphoblastic leukemia (ALL).
OBJECTIVE: To explore the immunophenotype and the changes of Treg in the peripheral blood of patients with ALL.
METHODS: The immunophenotype and levels of CD4+CD25+Treg in the peripheral blood were detected using flow cytometry in 35 ALL patients in comparison with the health control people.
RESULTS AND CONCLUSION: There were 22 B-ALL cases and 13 T-ALL cases among 35 ALL patients. CD19 expression of the cases of B-ALL had the highest positive rate (100%); meanwhile, CD7 expression of the cases of T-ALL had the highest positive rate (100%). There were no significant difference between the number of CD4+CD25+ Treg cells in B-ALL and T-ALL patients (P > 0.05), but there were significant difference between the number of Treg cells in health control people and ALL patients (P < 0.05). The results show that CD19 expression of the cases of B-ALL and CD7 expression of the cases of T-ALL had the highest positive rate; the levels of CD4+CD25+ Treg cells significantly increased in the peripheral blood of ALL patients.

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