Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8463-8468.doi: 10.3969/j.issn.1673-8225.2011.45.023

Previous Articles     Next Articles

Intravenous transplantation of bone marrow mesenchymal stem cells in the treatment of spinal cord injury in rats

Yu Chang-shen1,Guan Ya-lin2, Wang Shi-min2, Xu Xiao-lin2, Wang Xin-ping2, Yan Xiao-ling3, Zhang Xue-qing4   

  1. 1Postgraduate College, Tianjin Medical University, Tianjin  300070, China; 2Department of Neurology, 3Department of Pathology, 4Department of Neurophysiology, Huanhu Hospital of Tianjin, Tianjin  300060, China
  • Received:2011-08-04 Revised:2011-09-14 Online:2011-11-05 Published:2011-11-05
  • Contact: Wang Shi-min, Professor, Master’s supervisor, Department of Neurology, Huanhu Hospital of Tianjin, Tianjin 300060, China wangxinpingtj@sina.com
  • About author:Yu Chang-shen★, Studying for master’s degree, Postgraduate College, Tianjin Medical University, Tianjin 300070, China Yalin82@qq.com
  • Supported by:

    the Science and Technology Foundation of Tianjin Health Bureau, No. 05KY17*

PDF

394

Abstract:

BACKGROUND: Transplantation of stem cells can rebuild the structure and function of injured central nervous system, and has attracted wide attention in recent years.
OBJECTIVE: To explore the effects and mechanisms of bone marrow mesenchymal stem cells (BMSCs) transplanted through the vena caudalis on neurological recovery of rats with spinal cord injury.
METHODS: BMSCs were separated with density gradient centrifugation and adherent screening methods. Forty adult female Wistar rats were used to establish the models of spinal cord injury using aneurysm clips, and then randomized into two groups: control group and BMSCs transplantation group.
RESULTS AND CONCLUSION: The BMSCs transplantation group showed higher BBB scores than the control group (P < 0.05). Since the 30th day after injury, the motor evoked potential latency and somatosensory evoked potential P1 wave latency of the BMSCs transplantation group were shorter than those of the control group (P < 0.05), and these phenomena continued until the end of experiment. Positive cells could be noted at the sites 1 cm distant from the injured centre of the spinal cord. BMSCs transplantation via the vena caudalis can remarkably promote the recovery of neural function in rats with spinal cord injury, and the mechanism may be related to that transplanted BMSCs can survive, migrate and differentiate into neuron like cells and neuroglia like cells, and can promote the expression of neurotrophic factors.

CLC Number: