Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8424-8428.doi: 10.3969/j.issn.1673-8225.2011.45.014

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Human umbilical cord derived mesenchymal stem cell transplantation for rat traumatic brain injury

Yuan Yuan1, Yang Shu-yuan2, Zhang Jian-ning2   

  1. 1Department of Neurosurgery, Yantai Yu Huang Ding Hospital, Yantai  264000, Shandong Province, China
    2Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin  300040, China
  • Received:2011-05-02 Revised:2011-06-27 Online:2011-11-05 Published:2011-11-05
  • About author:Yuan Yuan☆, Doctor, Attending physician, Department of Neurosurgery, Yantai Yu Huang Ding Hospital, Yantai 264000, Shandong Province, China
  • Supported by:

    the Science and Technology Development Program of Yantai City, No. 2007139-4*

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Abstract:

BACKGROUND: There are rare reports about umbilical cord derived mesenchymal stem cells (UCMSCs) transplantation in the treatment of brain injury.
OBJECTIVE: To investigate the effects and mechanism of UCMSCs transplantation on the repair of rat brain fluid percussion injury.
METHODS: UCMSCs were separated from new-born umbilical cord and cultured in vitro, labeled with BrdU, and transplanted into rat brain 24 hours after fluid percussion injury. There were four groups: UCMSCs transplantation group, in situ UCMSCs transplantation; control group, injected with the same volume of DMEN/F12 medium; model group, without treatment; sham-injury group, no fluid percussion injury was made.
RESULTS AND CONCLUSION: Significant recovery of behavior was found in UCMSCs-treated rats at 1-3 weeks after transplantation. Immunohistochemical analysis showed that a small number of transplanted cells expressed neuron-specific enolase and glial fibrillary acidic protein. Compared with the control group, the expression of vascular endothelial growth factors increased in the injured region and the number of apoptotic cells decreased in the UCMSCs transplantation group. These findings show that UCMSCs transplantation can promote the early function recovery following brain fluid percussion injury through stimulating the secretion of vascular endothelial growth factors, increasing the number of microvessels in the injured region, and inhibiting cell apoptosis.

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