Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8413-8417.doi: 10.3969/j.issn.1673-8225.2011.45.012

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Effects of multi-intravenous transplantation of human cord blood mononuclear cells on the remodeling of cardiac collagen after myocardial infarction in rabbits

Li Wen-bin1,2, Yu Guo-long1, Yuan Chun-ju1, Yang Tian-lun1   

  1. 1Department of Cardiology, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
    2Department of Cardiology, Xiangtan Central Hospital, Xiangtan  411100 , Hunan Province, China
  • Received:2011-04-07 Revised:2011-06-10 Online:2011-11-05 Published:2011-11-05
  • Contact: Yu Guo-long, Doctor, Professor, Master’s supervisor, Department of Cardiology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China yuguolong123@yahoo.com.cn
  • About author:Li Wen-bin★, Master, Physician, Department of Cardiology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China; Department of Cardiology, Xiangtan Central Hospital, Xiangtan 411100 , Hunan Province, China

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Abstract:

BACKGROUND: In previous studies, the treatment of myocardial infarction by using stem cell transplantation was done via single intravenous or coronary injection transplantation.
OBJECTIVE: To investigate the effects of multi-intravenous transplantation of human cord blood mononuclear cells (HCBMCs) on the remodeling of cardiac collagen and heart function after myocardial infarction in rabbits.
METHODS: Forty-five Chinese rabbits underwent ligation of the left anterior coronary artery for the preparation of myocardial infarction models. They were randomly divided into three groups. ①Multi-transplantation group: They were intravenously injected with HCBMCs labeled with bromodexyuridine (BrdU) and normal saline at 7, 9, 11 and 13 days post-operation. ②Single cell transplantation group: They were intravenously injected with HCBMCs labeled with BrdU at 7 days post-operation and normal saline at 9, 11, and 13 days post-operation. ③Control group: They were intravenously injected with normal saline at 7, 9, 11 and 13 days post-operation. In addition, five Chinese rabbits acted as the sham operation group.
RESULTS AND CONCLUSION: Compared with the control group, left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) were improved significantly in the multi-transplantation group and single cell transplantation group (P < 0.05); while LVFS and LVEF were improved more significantly in the multi-transplantation group than in the single-transplantation group (P < 0.05); immunohistochemistry results demonstrated that BrdU positive cells were found in the peri-myocardial infarction area in the both transplanted groups at 2 and 4 weeks after the operation. Moreover, the number of BrdU positive cells in the multi-transplantation group was larger than that in the single-transplanted group. Masson's trichrome staining showed that compared with the sham operation group, the density of collagen fibers in the control group was significantly increased, and the collagen fibers were fused partially and arranged irregularly. Compared with the control group, the collagen and collagen fibers in both transplanted groups were decreased significantly, and the collagen fibers arranged more regularly after the operation. The multi-intravenous transplantation of HCBMCs for myocardial infarction is superior in improving cardiac function and inhibiting myocardial collagen remodeling, compared to single intravenously transplanted HCBMCs.

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