Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8408-8412.doi: 10.3969/j.issn.1673-8225.2011.45.011

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Immunological characteristics of human umbilical cord derived mesenchymal stem cells transplanted in vivo and vitro

Ye Xiang-yang1, Xu Yan1, Bi Wei-wei1, Guo Li-bin1, Li Xiang-jun2, Liu Jian-guo3   

  1. 1Department of Orthopedics, Central Hospital of Siping City, Siping  136000, Jilin Province, China
    2Department of Pathology, College of Pharmacy, Jilin University, Changchun  130021, Jilin Province, China
    3Department of Bone and Joint, First Hospital of Jilin University, Changchun  130021, Jilin Province, China
  • Received:2011-04-20 Revised:2011-05-31 Online:2011-11-05 Published:2011-11-05
  • Contact: Liu Jian-guo, Doctor, Professor, Doctoral supervisor, Department of Bone and Joint, First Hospital of Jilin University, Changchun 130021, Jilin Province, China jgliu.2005@yahoo.com.cn
  • About author:Ye Xiang-yang☆, Doctor, Associate chief physician, Department of Orthopedics, Central Hospital of Siping City, Siping 136000, Jilin Province, China xyyegk@yahoo.cn

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Abstract:

BACKGROUND: Human umbilical cord derived mesenchymal stem cells (HUC-MSCs) have been confirmed to have the characteristics of immunologic tolerance in vitro, but there are no relevant allogenic research in vivo. The results and mechanism are lack of consistency.
OBJECTIVE: To cultivate HUC-MSCs and research the immunological characteristics of implanted HUC-MSCs in vivo and in vitro.
METHODS: HUC-MSCs were obtained by using tissue adhesion method. Mixed-leukocyte reaction (MLR) was performed to evaluate immunologic tolerance in vitro, flow cytometry applied to detect the changes of CD4 and CD8 expression as well as ratio of CD4 to CD8, and ELISA used to detect interleukin 2 (IL-2), IL-10, γ-interferon (IFN-γ) levels.
RESULTS AND CONCLUSION: MLR results showed a similar trend in the concentration changes of cytokines: the level of IL-10 slightly increased after MLR reaction (P < 0.05), while the level of IFN-γ decreased (P < 0.05). The ratio of CD4+/CD8+ in T cell subsets was decreased to a slight extent after cell transplantation, indicating that HUC-MSCs possessed the characteristics of allogeneic immunological tolerance and immunoloregulation effect.

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