Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8404-8407.doi: 10.3969/j.issn.1673-8225.2011.45.010

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Proliferation characteristics of tumor stem cells in MCF7 cell line

Huang Ming-wei1, Lu Yun-fei2   

  1. 1The Affiliated Tumors Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
    2the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-14 Revised:2011-06-01 Online:2011-11-05 Published:2011-11-05
  • Contact: Lu Yun-fei, Master, Chief physician, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Huang Ming-wei☆, Doctor, Attending physician, the Affiliated Tumors Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China hmw503@163.com
  • Supported by:

    Guangxi Graduate Education Innovation Program, No. 2008105981002D33*

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Abstract:

BACKGROUND: The hypothesis of tumor stem cells posited that tumor stem cells are the root of tumor proliferation constantly and closely related to tumor biological behaviors such as infiltration, metastasis and drug-resistant.
OBJECTIVE: To investigate the proliferation characteristics of tumor stem cells in MCF7 cell line in vitro by studying the tumorigenic-colony forming rate at single cell level.
METHODS: Tumor stem cells proportion in MCF7 cell line was detected at series timing after passaged culture by single-cell-transplanting and tumorigenic-colony forming assay. The proportion of the CD44+/CD24-/low subset was detected by flow cytometry at the same time.
RESULTS AND CONCLUSION: The proportion of tumor stem cells and the CD44+/CD24-/low subset in MCF7cell line both varied regularly after passaged culture. But the variation of CD44+/CD24-/low (from 36.84% to 81.95%) subset was more drastic than that of tumor stem cells proportion,which only fluctuated in a limited range from 38.54% to 47.39%. The proportion of tumor stem cells was steady in MCF7 cell line after passaged culture, owing to the property of tumor stem cells to modulate and balance the proportion by itself. CD44+/CD24-/low subset neither represented nor enriched tumor stem cells subset in MCF7 cell line.  

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