Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8395-8398.doi: 10.3969/j.issn.1673-8225.2011.45.008

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The growth of nasopharyngeal cancer stem cell SP18 affected by carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1

Quan Sheng1, 2, Zhuang Ying-zhi1, Zhang Zhi-wei3, Liu Zhong-yuan3, Jiang Hao1, Zhao Qiang4, Zhu Jian-si3, Dong Lin3   

  1. 1Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang  421001, Hunan Province, China
    2Hunan Environment- Biological Polytechnic College, Hengyang  421001, Hunan Province, China
    3Cancer Research Institute of Medical College, University of South China, Hengyang  421001, Hunan Province, China
    4Department of Pathology, the First Affiliated Hospital, University of South China, Hengyang  421001, Hunan Province, China
  • Received:2011-07-26 Revised:2011-09-07 Online:2011-11-05 Published:2011-11-05
  • Contact: Zhuang Ying-zhi, Chief physician, Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China 988696@qq.com Correspondence to: Zhang Zhi-wei, Master, Associate professor, Cancer Research Institute of Medical College, University of South China, Hengyang 421001, Hunan Province, China zhanghiweichina@yahoo.com.cn
  • About author:Quan Sheng, Lecturer, Department of Oncology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China; Hunan Environment- Biological Polytechnic College, Hengyang 421001, Hunan Province, China 22029735@qq.com
  • Supported by:

    Innovation Platform Open Foundation of Hunan Universities, No. 10K052*; the Key Discipline Construction Fund of Hunan Province, No. 2006-180*; Graduate Research and Innovation Project of Hunan Province, No. CX2010B380*; College Students Learning and Innovative Research Project of Hunan Province, No. CXSY-SJ-09007*

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Abstract:

BACKGROUND: Carboxy terminal activating region-3 of the Epstein-Barr virus encoded latent membrane protein 1 (LMP1-CTAR3) has been constructed in the previous studies. CTAR3 plays an important role in LMP1.
OBJECTIVE: To observe the effect of LMP1-CTAR3 on the growth of nasopharyngeal cancer stem cell SP18.
METHODS: We adopted the method of retroviruses infection and established the nasopharyngeal cancer stem cell SP18 of stable expression LMP1 and LMP1△232-351. Then, we made use of cell growth curve, plate clone formation and soft agar colony experiment to observe the growth of nasopharyngeal cancer stem cell SP18 affected by LMP1△232-351. Meanwhile, we detected the cycle of SP18-LMP1△232-351 cell by flow cytometry and calculated cellular proliferation index. In addition, Western-blot detection was used to determine phosphorylation JAK3 protein.
RESULTS AND CONCLUSION: Compared with SP18-LMP1 cells, the growth velocity and proliferation index of SP18-LMP1△232-351 cells obviously decreased (P < 0.01). The expression of phosphorylation JAK3 protein in SP18-LMP1△232-351 cells was lower than that in SP18-LMP1 cells. LMP1-CTAR3 probably affected the expression of phosphorylation JAK3 protein in SP18 to decrease cellular proliferation index and reduce the ability of cell proliferation promoted by LMP1.

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