Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8391-8394.doi: 10.3969/j.issn.1673-8225.2011.45.007

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Chondrogenic differentiation of rabbit adipose-derived stem cells in culture medium containing transforming factors and transferrin

Yang Guo-qing1, Wang Zhao-jie2, An Rong-ze2, Zhao Jun-yan2, Qi Xin-wen2   

  1. 1Department of Surgery, Meitan County Hospital, Zunyi  564100, Guizhou Province, China
    2Department of Orthopedics, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai  519100, Guangdong Province, China
  • Received:2011-07-29 Revised:2011-09-05 Online:2011-11-05 Published:2011-11-05
  • Contact: Wang Zhao-jie, Doctor, Chief physician, Professor, Department of Orthopedics, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai 519100, Guangdong Province, China zhaojiewang@163.com
  • About author:Yang Guo-qing, Associate chief physician, Department of Surgery, Meitan County Hospital, Zunyi 564100, Guizhou Province, China yangguoqing6666@sina.com
  • Supported by:

    Guizhou Science and Technology Bureau, No. [2006]62-2*; the Medical Key Foundation of Zhuhai City, No. [2008]80*

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Abstract:

BACKGROUND: The choice of seed cells is a key factor to the cartilage tissue engineering.
OBJECTIVE: To explore the chondrogenic potential of adipose-derived stem cells (ADSCs) in culture medium containing transforming factors and transferring.
METHODS: ADSCs were obtained from the subcutaneous adipose tissue of 6-month-old New Zealand white rabbits’ neck back by mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. The adhesion and growth of cells were observed using inverted phase contrast microscope. Two weeks after the cells were cultured in chondrogenic medium, collagen type Ⅱ expression was detected by immunohistochemistry staining.
RESULTS AND CONCLUSION: The primary stem cells isolated from adipose tissue adhered on the plate in 24 hours, and reached 90% confluence in single layers after 96 hours. The cartilage nodules were observed at 7 days after ADSCs were cultured under chondrogenic medium, while collagen type Ⅱ was positive 14 days later. ADSCs of rabbits can express chondrogenic phenotype when they were cultured in chondrogenic medium, which proves the ADSCs can be one of the options for cartilage tissue engineering.

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