Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8361-8365.doi: 10.3969/j.issn.1673-8225.2011.45.001

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Multipotent differentiation status of rat bone marrow mesenchymal stem cells in vitro after shear stress loading

Liu Lin1, Yang Si-yuan2, Hu Xuan-yi2, Lin Ke3, Shi Ying-kang3   

  1. 1Department of Respiratory Medicine, Guizhou Provincial People’s Hospital, Guiyang  50002, Guizhou Province, China
    2Department of Cardiac Surgery, the Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China
    3Department of Thoracic-Cardiac Surgery, West China Hospital, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2011-04-14 Revised:2011-05-26 Online:2011-11-05 Published:2011-11-05
  • Contact: Yang Si-yuan, Doctor, Associate chief physician, Department of Cardiac Surgery, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China wo418@tom.com
  • About author:Liu Lin★, Master, Attending physician, Department of Respiratory Medicine, Guizhou Provincial People’s Hospital, Guiyang 50002, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 10402026*; Guizhou Provincial Governor Special Fund for Scientific and Educational Talents, No. 200891*; Doctor Foundation of the Affiliated Hospital of Guiyang Medical College*

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Abstract:

BACKGROUND: The number of mesenchymal stem cells in the bone marrow of adult animals is little.
OBJECTIVE: To investigate whether shear stress could modify the abilities of growth, amplification and multi-potent differentiation of bone marrow mesenchymal stem cells or not.
METHODS: SD rat bone marrow mesenchymal stem cells were isolated, cultured and amplified in vitro by Percoll density gradient centrifugation and adherent culture. 1 Pa shear stress was loaded on primary passage mesenchymal stem cells with a flow chamber system for osteogenic and adipogenic differentiation.
RESULTS AND CONCLUSION: The primary cells were mostly spindle-like in shape, like the fibroblast cells, and formatted clones. After the cells were loaded with shear stress and then passaged, they grew faster and bigger, and had the same morphologic characters similar as primary mesenchymal stem cells. The growth curves showed the growth regularity of all passage cells was similar, the laser scanning confocal microscope showed the CD41 and CD90 were positive, CD31 and CD45 were negative. After the cells were induced for 14 days, the results of Von Kossa strain and oil red O strain were both positive. The abilities of growth, amplification and multi-potent differentiation of bone marrow mesenchymal stem cells maintained after shear stress loading.

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