Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (42): 7791-7795.doi: 10.3969/j.issn.1673-8225.2011.42.001

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Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein and transgene myoblasts in rabbits

Zhang Li1, 2, Wang Wei2   

  1. 1Liaoning University of Traditional Chinese Medicine, Shenyang  110032, Liaoning Province, China
    2Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou  121000, Liaoning Province, China
  • Received:2011-01-21 Revised:2011-04-18 Online:2011-10-15 Published:2011-10-15
  • Contact: Wang Wei, Doctor, Professor, Doctoral supervisor, Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou 121000, Liaoning Province, China Weiwang_Ly@ yahoo.com.cn
  • About author:Zhang Li☆, Studying for doctorate, Attending physician, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China; Fifth Clinical College of Liaoning Medical University (Jinzhou Central Hospital), Jinzhou 121000, Liaoning Province, China Zhangli2004jz@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772190*; the Innovation Team Project of Liaoning Educational Bureau, No. 2006 123-33*; Liaoning Provincial Science and Technology Program, No. 2005225003-14*

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Abstract:

BACKGROUND: In recent years it has been reported that ciliary neurotrophic factor (CNTF) can inhibit the myogenic differentiation of myoblast in vitro to maintain the dedifferentiated status of myoblasts. Myoblasts transfected with CNTF can be used as seed cells for tissue-engineered bone, which combined with bone morphogenetic protein (BMP) under certain conditions can promote the repair of bone defects.
OBJECTIVE: To investigate the effect of transfergene myoblasts in combination with BMP to repair radius defects in rabbits through sodium hyaluronate (HA) gel as a carrier.
METHODS: At aseptic conditions, about 1.0 cm segmental bone defect was produced in the middle of rabbit radius, which was bridged with a 1.4 cm silica tube. Different inserts were implanted into the tube: myoblasts transfected with CNTF and BMP using the HA gel as a carrier (experimental group); BMP only compounded with the HA gel (control group); only HA gel implant group (blank group).
RESULTS AND CONCLUSION: The animal experiments showed that the results of X-ray test, general observation, and pathohistological observation were better in the experimental group than the control and blank groups, and also better in the control group than the blank group. With GTR membrane as a silicone tube, HA gel compound transgenic myoblasts with BMP are feasible to construct a a new type tissue-engineered bone which has good effect on the repair of radius defects.

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