Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7689-7692.doi: 10.3969/j.issn.1673-8225.2011.41.022

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Effects of serum concentration on the proliferation of human cytokine induced killer cells

Ren Si-po, Han Guang-yu, Shi Li, Tan Kun, Geng Yue-chun, Yang Qin-xiang, Wang Jian   

  1. Xuzhou Institute of Medical Sciences, Xuzhou  221006, Jiangsu Province, China
  • Received:2011-05-05 Revised:2011-08-16 Online:2011-10-08 Published:2011-10-08
  • Contact: Wang Jian, Associate researcher, Xuzhou Institute of Medical Sciences, Xuzhou 221006, Jiangsu Province, China xzsyks@yahoo.com.cn
  • About author:Ren Si-po★, Master, Technician in charge, Xuzhou Institute of Medical Sciences, Xuzhou 221006, Jiangsu Province, China rensipo@163.com
  • Supported by:

    Social Development Program of Bureau of Science and Technology of Xuzhou City, No.XZ2006268*; XM09B004*

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Abstract:

BACKGROUND: Cytokine induced killer (CIK) cells are cultured mostly in 10% serum, but there have been few studies describing the effects of serum concentration on growth characteristics of CIK cells.
OBJECTIVE: To investigate the proliferation of CIK cells in different concentrations of serum.
METHODS: CIK cells were cultured with 10%, 20% and 30% fetal bovine serum supplemented with recombinant human interleukin (IL-2). The effects of serum concentration on cell cycle were analyzed using flow cytometry. The proliferation of CIK in different concentrations of serum was determined by MTT assay and the growth characteristics of CIK cells were observed.
RESULTS AND CONCLUSION: The proliferation rate and proliferation index of CIK cells were the lowest in 10% serum and highest in 30% serum. There was statistical significance in proliferation rate among three groups (P < 0.05 or P < 0.01). The proliferation of CIK cells cultured are serum concentration-dependent in vitro.

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