Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7597-7600.doi: 10.3969/j.issn.1673-8225.2011.40.044

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CD133 expression in peripheral blood cells of patients with colorectal cancer 

Huang Ying-feng1, Wei Ya-ming2, Li Qiu-xian2, Nie Yu-qiang3, Gu Wei-li1   

  1. 1General Surgery Department, 2Blood Transfusion Department, 3Department of Gastroenterology and Hepatology, the First People’s Hospital of Guangzhou, Guangzhou  510180, Guangdong Province, China
  • Received:2011-02-21 Revised:2011-05-18 Online:2011-10-01 Published:2011-10-01
  • Contact: Wei Ya-ming, Doctor, Researcher, Chief technician, Blood Transfusion Department, the First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China weiyaming@163.com
  • About author:Huang Ying-feng★, Master, Chief physician, General Surgery Department, the First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China
  • Supported by:

    Guangdong Provincial Medical Research Foundation, No. A2008517*; Guangzhou Medical and Health Science and Technology Program, No. 2007-YB-004*; Guangzhou Applied Basic Project, No. 2008J1-C171*

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Abstract:

BACKGROUND: It has been reported recently that a higher CD133 positive expression in the peripheral blood of cancer patients was detected.
OBJECTIVE: To investigate the expression and clinical value of CD133 mRNA in the peripheral blood of patients with colorectal cancer.
METHODS: The flow cytometry method and RT-PCR were used to detect the expression of CD133 mRNA and protein in peripheral blood samples which were from 20 controls and 29 patients with colorectal cancer.
RESULTS AND CONCLUSION: The CD133+ cell percentages in both peripheral blood samples of controls and colorectal cancer patients without metastasis were lower than those of colorectal cancer patients with metastasis (P < 0.01). RT-PCR results revealed 5 cases of positive CD133 mRNA expression in all 14 colorectal cancer patients with metastasis, and the positive ratio was 35.7%, in which run more than 38 PCR circles. The expression of CD133 in peripheral blood cells of colorectal cancer patients with metastasis is higher than that of normal controls and colorectal cancer patients without metastasis.

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