Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7105-7108.doi: 10.3969/j.issn.1673-8225.2011.38.017

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Immunodetection to Escherichia coli of polydiacetylene nanoparticles

Guo Xun-zhi1,2, He Jian-tai1, Zhang Yang-de1, Zhou Wei-hua2   

  1. 1National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha  410008, Hunan Province, China
    2 Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China
  • Received:2011-06-16 Revised:2011-07-11 Online:2011-09-17 Published:2011-09-17
  • Contact: Zhou Wei-hua, Associate professor, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China subdeb@163.com
  • About author:Guo Xun-zhi☆, Studying for doctorate, Lecturer, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China；Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China
  • Supported by:

    the Science and Technology Project of Jiangxi Education Bureau, No. GJJ09359*; the Scientific Supporting Program of Jiangxi Province, No. 2009BSA12400*

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Abstract:

BACKGROUND: PCR, ELISA, immunofluorescence-ball method, and gene chip technology for rapid detection of enteric pathogens take hours to get results and are not suitable for on-site rapid test.
OBJECTIVE: To prepare polydiacetylene (PDA) nanoparticles for immunodetection to Escherichia coli.
METHODS: PDA nanoparticles were prepared with sonication.
RESULTS AND CONCLUSION: The prepared PDA nanoparticles had uniform particle size, narrow distribution, and stable structure. Escherichia coli dilution was dropped into PDA nanoparticle dilution for 2 minutes, and the solution color turned from blue to red, indicating that PDA nanoparticles were rapid in detection speed. The graphs of ultraviolet-visible absorption spectroscopy, transmission electron microscope, and nanosizer displayed big changes with Escherichia coli and antibody PDA nanoparticle. The experimental results signed that the rat polyclonal antibody is a functional molecule of the immunodetection to Escherichia coli of PDA nanoparticles, and the reaction of antigen-antibody is specific.

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