Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7100-7104.doi: 10.3969/j.issn.1673-8225.2011.38.016

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Modified poly (L-lactic acid)-poly (L-lysine) polymer induces bone marrow stromal cells initial adhesion

Mao Xue-li1, Ling Jun-qi1, Xiao Yin2, Peng Hui3   

  1. 1Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou  510055, Guangdong Province, China
    2Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia
    3Centre for Magnetic Resonance, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia
  • Received:2011-06-30 Revised:2011-07-26 Online:2011-09-17 Published:2011-09-17
  • Contact: Ling Jun-qi, Professor, Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong Province, China lingjq@ mai.sysu.edu.cn
  • About author:Mao Xue-li☆, Doctor, Lecturer, Department of Operative Dentistry and Endodontics, Stomatology Hopital of Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong Province, China maoxuel@mail.sysu.edu.cn
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 10451008901005929*

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Abstract:

BACKGROUND: It is an effective strategy that integrating the activated ligands into the surface of biomaterial scaffolds to enhance and regulate the material affinity for cells and cell compatibility.
OBJECTIVE: To develop poly (L-lactic acid)-poly (L-lysine) (PLLA-PLL) to enhance cell initial adhesion on the polymer surface.
METHODS: Different composition polymer films (PLLA139-PLL131, PLLA77-PLL72, PLLA45-PLL246) were synthesized through ring-opening metathesis polymerization. BMSCs were seeded onto these different composition polymer surfaces ( PLLA and TCP served as the control), and detected the best composition of PLLA-PLL polymer film for cell adhesion and proliferation.
RESULTS AND CONCLUSION: PLLA-PLL films increased the number of initially attached BMSCs in both serum and serum free conditions compared with PLLA. Increased PLL content in the PLLA-PLL co-polymer led to increase cell attachment, significant difference changes were detected on copolymer PLLA77-PLL72 (P< 0.05). Continued culture confirmed that PLLA77-PLL72 enhanced cell proliferation. BMSCs cultured on the PLLA-PLL had a clear and well organized F-actin fibre expression.

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