Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7095-7099.doi: 10.3969/j.issn.1673-8225.2011.38.015

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Chitosan hydrogel microsphere-templated microcapsules carrying heparin via layer-by-layer self-assembly

Wu Ying-feng1, Guo Hai-li2, Li Juan2   

  1. 1Department of Vascular Surgery, Xuanwu Hospital & Institute of Vascular Surgery, Capital Medical University, Beijing  100053, China
    2CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing  100049, China
  • Received:2011-01-14 Revised:2011-03-05 Online:2011-09-17 Published:2011-09-17
  • Contact: 李娟，博士，助理研究员，主要从事微纳米药物载体和组织工程材料的生物相容性研究。中国科学院高能物理研究所，北京市 100049
  • About author:Wu Ying-feng☆, Doctor, Attending physician, Department of Vascular Surgery, Xuanwu Hospital & Institute of Vascular Surgery, Capital Medical University, Beijing 100053, China wyf799@hotmail.com
  • Supported by:

    the Natural Science Foundation of Beijing, No. 2103049*, 5082007*; Beijing Science and Technology Committee Foundation, No. Y0905001000091*; Graduate Innovation Fund of Beijing Institute of Technology, No. GA200808*

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Abstract:

BACKGROUND: Electrostatic layer-by-layer (LbL) self-assembly is a simple, effective and novel approach to carrying macromolecules into microcapsule carriers.
OBJECTIVE: To prepare chitosan (CS) microcapsules to control the releasing of heparin (Hep) by LbL.
METHODS: CS microspheres were fabricated by a sodium sulfate-based precipitation process and then used as positively charged templates for polyelectrolyte multilayer coatings to carry heparin by the LbL self-assembly technique. The polycation was CS and the polyanion was Hep. The microcapsule was formed via coating {CS/Hep}3 on CS hydrogel template. The microcapsules composed of {CS/ Hep}3 on CS hydrogel templates were confirmed by fluorescence inverted microscopy, confocal laser scanning microscopy and laser particle size analysis. The assembly of polyelectrolyte layers on CS hydrogel microspheres was monitored by Zeta Potential Analyzer.
RESULTS AND CONCLUSION: The resulting microcapsules were about 1 μm in average diameter, and allowed spontaneous loading of heparin through electrostatic interaction, with the encapsulation efficiency and carrying capacity of 83.8% and 3.05%, respectively.

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