Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6932-6936.doi: 10.3969/j.issn.1673-8225.2011.37.022

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Effects of RNA interference on interleukin-6 expression in endothelial cells and migration of vascular smooth muscle cells

Wang Gang1, 2, Liu Chao-zhong1, Song Yun-long3, Zhang Xiao-hui4, Zhang Li4, Li Li4, Cao Jun-han5, Zhang Hong-chao4   

  1. 1Department of Cardiovascular Medicine, 3CR/MRI Room, 4Department of Cardiovascular Surgery, PLA  Airforce General Hospital, Beijing 100142, China; 2Department of Cardiovascular Medicine, the 452 Hospital of Chinese PLA, Chengdu 610041, Sichuan Province, China; 5Department of Sichuan Industrial Institute of Antibiotics Co., Ltd., Chengdu 610000, Sichuan Province, China
  • Received:2011-03-19 Revised:2011-06-09 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Hong-chao, Doctor, Associate chief physician, Master’s supervisor, Department of Cardiovascular Surgery, Airforce General Hospital, Beijing 100142, China zhanghc2002@ gmail.com
  • About author:Wang Gang★, Master, Physician, Department of Cardiovascular Medicine, PLA Airforce General Hospital, Beijing 100142, China; Department of Cardiovascular Medicine, the 452 Hospital of Chinese PLA, Chengdu 610041, Sichuan Province, China wg1002@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872539*

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Abstract:

BACKGROUND: Inflammatory reaction easily occurs in target vascular region after implantation of intravascular stent.
OBJECTIVE: To investigate the effects of siRNA technique on migration of vascular smooth muscle cells by inhibiting the expression of interleukin-6 (IL-6) in the endothelial cells.
METHODS: The time gradient and concentration gradient of the IL-6 mRNA expression in the EA.HY926 cells stimulated by lipopolysaccharide (LPS) were detected by RT-PCR. Short hairpin RNA eukaryotic expression vector targeting IL-6 gene, named pGensil-1.1-IL-6, was constructed and transfected into EA.HY926 cells for intervention of IL-6 expression by lipofectamine 2000.
RESULTS AND CONCLUSION: After pGensil-1.1-IL-6 was transfected into EA.HY926 cells, IL-6 mRNA and protein expression in the EA.HY926 cells stimulated by LPS was obviously decreased. In co-culture model, under the effect of EA.HY926 cells transfected by pGensil-1.1-IL-6, matrix metalloproteinase 9 mRNA and protein expression in the human cord venous smooth muscle cells was obviously decreased. The number of human cord venous smooth muscle cells was decreased. These findings suggest that siRNA technique can inhibit the expression of IL-6 in endothelial cells and attenuate the migration of smooth muscle cells by decreasing matrix metalloproteinase 9 expression.

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