Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6909-6912.doi: 10.3969/j.issn.1673-8225.2011.37.017

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Expression identification and preliminary function analysis of S100a7 in retinal pigment epithelial cells cultured in vitro

Xu Guang-jun, Zhang Xue-dong, Chen Xue-mei   

  1. Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China
  • Received:2011-03-09 Revised:2011-03-24 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Xue-dong, Professor, Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China zxued@sina.com
  • About author:Xu Guang-jun★, Studying for master’s degree, Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China 308855959@qq.com

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Abstract:

BACKGROUND: S100a7 is a new protein that participates in cell proliferation, vascularization and other pathophysiological processes.
OBJECTIVE: To discuss the expression of S100a7 in retinal pigment epithelial cell (RPE) and to investigate the effects of S100a7 on RPE proliferation.
METHODS: Immunofluorescence and western blot techniques were used to detect the expression of S100a7 in ARPE-19, and MTT was performed to investigate the effects of S100a7 on RPE proliferation.
RESULTS AND CONCLUSION: S100a7 was proven to be expressed in ARPE-19 by immunofluorescence and western blot techniques and it could significantly promote ARPE-19 cell proliferation. This study first confirmed the expression of S100a7 in ARPE-19 cells, which may play a specific role in the pathogenesis of proliferative vitreoretinopathy.

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