Abstract:

BACKGROUND: Vascular smooth muscle cell proliferation is the basis for changes of cardiovascular tissue.
OBJECTIVE: To investigate the effects of different concentrations of cigarette smoke extract on the expression of connective tissue growth factor in rat aortic smooth muscle cells.
METHODS: Passage 3 rat aortic smooth muscle cells were interfered with 2.5%, 5%, 10% and 20% cigarette smoke extract.
RESULTS AND CONCLUSION: Real-time quantitative PCR results showed that treatment with 2.5%, 5%, 10% and 20% cigarette smoke extract for 12 hours can increase mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, in particular 5% cigarette smoke extract, and that mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells was dose-dependent within 24 hours. Results demonstrated that cigarette smoke extract can upregulate the mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, leading to the proliferation of rat aortic smooth muscle cells.