Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6879-6882.doi: 10.3969/j.issn.1673-8225.2011.37.010

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Effects of recombinant human tumor necrosis factor receptor type Ⅱ antibody fusion protein on serum troponin Ⅰ and tumor necrosis factor alpha expression in myocardial tissue in a rat model of myocardial ischemia/reperfusion injury

Chen Yan1, Xia Hao1, Bao Cui-yu2, Chen Hong-guang2, Wang Xin-liang2, Xie Hua-qiang1, Liu Lan1   

  1. 1Remin Hospital of Wuhan University, Wuhan   436000, Hubei Province, China
    2Research Center for Cardiology, Xianning College, Xianyang   437100, Hubei Province, China
  • Received:2011-02-26 Revised:2011-03-14 Online:2011-09-10 Published:2011-09-10
  • Contact: Bao Cui-yu, Doctor, Chief physician, Professor, Master’s supervisor, Research Center for Cardiology, Xianning College, Xianning 437100, Hubei Province, China bcy_tiaopi@126.com
  • About author:Chen Yan★, Studying for master’s degree, Remin Hospital of Wuhan University, Wuhan 436000, Hubei Province, China Chenyan99188@ 163.com
  • Supported by:

    the Natural Science Foundation of Hubei Province, No. 2008CDZ084*; Youth Program of Education Department of Hubei Province, No. Q20082801*

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Abstract:

BACKGROUND: A lot of tumor necrosis factor α (TNF-α) generating during myocardial ischemia/reperfusion can cause worsened myocardial systolic function.
OBJECTIVE: To investigate the effects of recombinant human tumor necrosis factor receptor typeⅡantibody fusion protein on myocardial tissue in a rat model of myocardial ischemia/reperfusion injury.
METHODS: Myocardial ischemia/reperfusion injury was induced in adult male Wistar rats. In the drug intervention group, recombinant human tumor necrosis factor receptor typeⅡantibody fusion protein was injected before reperfusion. In the model group, normal saline was given before reperfusion. A sham-operated group with myocardial ischemia/reperfusion injury induction was set. Immediately after reperfusion, myocardial infarction area was measured, TNF-α and serum cardiac troponin Ⅰwere detected by ELⅠSA, and TNF-α mRNA expression was determined by real time-PCR.
RESULTS AND CONCLUSION: Compared with sham-operated group, TNF-α, TNF-α mRNA, and serum troponinⅠ evels were significantly increased in the model and drug intervention groups (P < 0.05). Compared with model group, TNF-α and serum troponinⅠlevels, myocardial infarction area and TNF-αmRNA expression were significantly decreased (P  < 0.05), in the drug intervention group. Results showed that recombinant human tumor necrosis factor receptor type Ⅱ antibody fusion protein can alleviate myocardial ischemia/reperfusion injury and improve cardiac function in rats.

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