Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6871-6874.doi: 10.3969/j.issn.1673-8225.2011.37.008

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Concentrations of transforming growth factor beta 1 in rabbit aqueous humor

Wu Wei1, Liao Guo-ping2, Lu Xiao-he1, Liang Li-fang1, Yan Li-meng1, Chen Han 1, Zhang Hai-feng1, Xu Wei1, Wei Er-xia1   

  1. 1Department of Ophthalmology, 2Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-04-23 Revised:2011-08-01 Online:2011-09-10 Published:2011-09-10
  • Contact: Lu Xiao-he, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 515282, Guangdong Province, China luophthal@126.com
  • About author:Wu Wei★, Studying for master’s degree, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Wuwei200414952@ 126.com

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Abstract:

BACKGROUND: Studies has demonstrated that transforming growth factor β1(TGF-β1) eyedrops is not only involved in the tissue metabolism of normal eye as well as kinds of physiological function, but also plays an important role in the treatment effect on corneal wound healing, uveitis and nearsightedness .
OBJECTIVE: To investigate the concentration of TGF-β1 in aqueous humor after ophthalmic administration and to establish the method for quantitatively analyzing the TGF-β1 levels in rabbit aqueous humor.
METHODS: Thirty rabbits were randomly divided into blank control, TGF-β1 0.5, 1.0, 2.0, 4.0 mg/L groups, with six animals in each group. PBS and 0.5, 1.0, 2.0,4.0 mg/L TGF-β1 were dropped into the right eyeballs four times per day for 1 week. 
RESULTS AND CONCLUSION: No conjunctival secretion, bulbar conjunctiva hyperemia, corneal edema, keratic precipitates, inflammation in anterior chamber, or vitreous haze lens opacity was found prior to and following experiments in each group. ELISA results showed that compared with PBS, both 2.0 mg/L and 4.0 mg/L TGF-β1 eyedrops can significantly improve the TGF-β1 concentration effectively in rabbit aqueous humor (P < 0.05), which has a good penetrating ability in the cornea, and can effectively enhance treatment concentration in aqueous humor.

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