Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6863-6865.doi: 10.3969/j.issn.1673-8225.2011.37.006

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Morphological and immunohistochemical detection of deep-frozen bone

Xia Wu-xian, Gou Hua, Li Long-teng, Han Fen, Sun Yong-wu, Zhang Xiang-jun, Zhang Fan   

  1. Zhengzhou Railway Vocational Technical College, Zhengzhou 450052, Henan Province, China
  • Received:2011-01-21 Revised:2011-02-11 Online:2011-09-10 Published:2011-09-10
  • About author:Xia Wu-xian, Associate professor, Zhengzhou Railway Vocational Technical College, Zhengzhou 450052, Henan Province, China xiawuxian@yahoo. com.cn
  • Supported by:

    the Natural Science Research Plan Program of Education Department of Henan Province, No. 2009C310014*

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Abstract:

BACKGROUND: Bone morphogenetic protein 2 (BMP-2) in fracture healing and repair process plays an important role in deep-freezing technology.
OBJECTIVE: To investigate the effects of deep-freezing technology on BMP-2 expression in the bone materials.
METHODS: 18 rabbits were randomly and evenly divided into three groups: deep-frozen 3 months, deep frozen 6 months, fresh bone. The middle upper 1/3 right tibia from each group was taken and preserved at -80 ℃ for 3 or 6 months or was not preserved. Then all bone samples were performed hematoxylin-eosin staining and BMP-2 immunohistochemical staining.
RESULTS AND CONCLUSION: There was no significant difference in morphology between deep frozen bone and fresh bone. BMP-2 was expressed in bone deep-frozen for 3 and 6 months, but the expression was lower than that in the fresh bone.

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