Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6859-6862.doi: 10.3969/j.issn.1673-8225.2011.37.005

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Porcine brain gray matter tissue extract promotes the proliferation of rat osteoblasts cultured in vitro

Xia Xin-lei1, Huang Gang-yong1, Ma Xin1, Gong Xiao-kang1, Jin Wei-fang2, Huang Huang-yuan1, Jiang Jian-yuan1   

  1. 1Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai  200040, China
    2Laboratory of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai   200032, China
  • Received:2011-05-05 Revised:2011-07-21 Online:2011-09-10 Published:2011-09-10
  • Contact: Ma Xin, Doctor, Professor, Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, China maxin@sh163.net
  • About author:Xia Xin-lei☆, Doctor, Attending physician, Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, China cormierhuang@ yahoo.com.cn

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Abstract:

BACKGROUND: Evidence exists that rat brain tissue extract can promote the proliferation of rat osteoblasts and bone marrow stromal stem cells.
OBJECTIVE: To investigate the effects of porcine brain inherent component extract on proliferation of rat osteoblasts cultured in vitro.
METHODS: Porcine and rat brain gray matter, white matter, pituitary gland, limb skeletal muscle were harvested to prepare into homogenate. A blank control group was set. Extract from each tissue was used to culture rat osteoblasts. Effects of each tissue extract on osteoblasts were compared.
RESULTS AND CONCLUSION: Compared with blank control group, porcine and rat brain gray matter, white matter, and pituitary gland all significantly promoted the proliferation of osteoblasts (P < 0.05). Porcine and rat brain gray matter extract better promoted the proliferation of osteoblasts than white matter and pituitary gland extract (P < 0.05). Results showed that porcine brain tissue extract can promote the proliferation of rat osteoblasts, and porcine brain gray matter produced the most significant promoting effects.

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